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Hampton HR2-643 Parabar 10312 Hampton代理
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Parabar 10312 (previously known as Paratone)
HR2-643 Parabar 10312 100 ml bottle
HR2-862 Parabar 10312 5 ml bottle
Applications
Oil based cryoprotectant
Features
Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
Oil based cryoprotectant
Description
Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1 Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.
HR2-643 Parabar 10312 is supplied as 100 ml in an amber glass bottle, no applicator rod.
HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.
The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.
Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.
Refractive index 1.48487 at 20 degrees Celsius.
Kinematic viscosity 641 cSt at 100 degrees Celsius. Kinematic viscosity ratio 2.05 at 100 degrees Celsius.
Clear to slightly hazy is appearance.
Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
Paratone® is a registered trademark of Chevron Corporation.
CAT NO | NAME | DESCRIPTION |
HR2-643 | Parabar 10312 | 100 ml bottle, each |
HR2-862 | Parabar 10312 | 5 ml bottle with applicator rod, each |
References | ||
1. | H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26. | |
2. | Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071 | |
3. | H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126 | |
4. | S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953 |
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